Lepr^tm3.2Rck
Targeted Allele Detail

Summary

• Symbol: Lepr^tm3.2Rck
• Name: leptin receptor; targeted mutation 3.2, Jeffrey M Friedman
• MGI ID: MGI:5698840
• Synonyms: ObRa KO
• Gene: Lepr Location: Chr4:101574601-101672549 bp, + strand Genetic Position: Chr4, 46.96 cM
• Alliance: Lepr^tm3.2Rck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221309
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: Two loxP sites were inserted upstream and downstream of the coding region of 19a. A PGK-Neo cassette, flanked by two FRT sites was inserted upstream of 19a in intron 18 next to the loxP site. The neomycin selection cassette was removed via Flp-mediated recombination. Exon 19a was deleted by Cre-mediated recombination. (J:221309)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lepr Mutation: 123 strains or lines available

References

• Original: J:221309 Li Z, et al., Phenotypic effects of an induced mutation of the ObRa isoform of the leptin receptor. Mol Metab. 2013;2(4):364-75
• All: 1 reference(s)