Lhx1^tm2.1Rob
Targeted Allele Detail

Summary

• Symbol: Lhx1^tm2.1Rob
• Name: LIM homeobox protein 1; targeted mutation 2.1, Elizabeth J Robertson
• MGI ID: MGI:5698528
• Synonyms: Lhx1^CA
• Gene: Lhx1 Location: Chr11:84409110-84416361 bp, - strand Genetic Position: Chr11, 51.31 cM
• Alliance: Lhx1^tm2.1Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226614
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Constitutively active)
• Mutation: Insertion
• Mutation details: Using vector PGRS0002_B_E08, the L1L2_Bact_P cassette was inserted at position 84522718 of Chromosome 11 upstream of the critical exons 2 and 3 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 and 3 at position 84521461. The critical exons 2 and 3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:226614)

Expression

In Mice Carrying this Mutation:	30 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lhx1 Mutation: 22 strains or lines available

References

• Original: J:226614 Costello I, et al., Lhx1 functions together with Otx2, Foxa2, and Ldb1 to govern anterior mesendoderm, node, and midline development. Genes Dev. 2015 Oct 15;29(20):2108-22
• All: 1 reference(s)