Actb^tm2.1Kftnk
Targeted Allele Detail

Summary

• Symbol: Actb^tm2.1Kftnk
• Name: actin, beta; targeted mutation 2.1, Kenji Tanaka
• MGI ID: MGI:5637315
• Synonyms: Actb^{tm2.1(tetO-YCnano50)Kftnk}, tetO-YC-Nano50
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^tm2.1Kftnk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:212077
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6 x 129

Mutation description

• Allele Type: Targeted (Inducible, No functional change, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: A construct comprising the tetO tetracycline response element, rabbit beta-globin intron, Yellow Cameleon Nano50, SV40 poly(A) signal sequence and an FRT site flanked neomycin resistance gene cassette, was inserted 799 bp downstream of the Actb poly(A) signal. The neo cassette was removed through subsequent flp-mediated recombination. Yellow Cameleon Nano50 is a Forster resonance energy transfer (FRET)-based genetically encoded Ca2+ indicator (GECI) with a +3 linker (Gly-Gly-Ser) between the calmodulin motif and the M13 peptide. The inclusion of the longer linker segment increases Ca2+ affinity (Kd = 50 nM). (J:212077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 51 strains or lines available

Notes

ES cell line = G0G1

References

• Original: J:212077 Kanemaru K, et al., In Vivo visualization of subtle, transient, and local activity of astrocytes using an ultrasensitive ca(2+) indicator. Cell Rep. 2014 Jul 10;8(1):311-8
• All: 17 reference(s)