Map3k5^tm2.1Hijo
Targeted Allele Detail

Summary

• Symbol: Map3k5^tm2.1Hijo
• Name: mitogen-activated protein kinase kinase kinase 5; targeted mutation 2.1, Hidenori Ichijo
• MGI ID: MGI:5617541
• Synonyms: ASK1^ASKA
• Gene: Map3k5 Location: Chr10:19810218-20018499 bp, + strand Genetic Position: Chr10, 9.35 cM
• Alliance: Map3k5^tm2.1Hijo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:210627
• Parent Cell Line: CMTI-2 (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Mutations (V745L, M761Aand S828A) were introduced into the ATP-binding pocket of the kinase to create an analog sensitive kinase allele and a floxed neo cassette was inserted in intron 17 via homologous recombination. Cre mediated recombination removed the neo cassette. Kinase activity of this allele can be controlled by the administration of 1Na-PP1, a derivative of the general kinase inhibitor PP1. (J:210627)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map3k5 Mutation: 63 strains or lines available

References

• Original: J:210627 Mizukami J, et al., ASK1 promotes the contact hypersensitivity response through IL-17 production. Sci Rep. 2014;4:4714
• All: 2 reference(s)