Hprt1^{tm1(CAG-Glra3*)Jcme}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(CAG-Glra3*)Jcme}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Jochen C Meier
• MGI ID: MGI:5608589
• Synonyms: Hprt^alpha3L185L, Hprt^{tm1(CAG-Glra3*)GW}
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(CAG-Glra3*)Jcme} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207981
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Hprt1^{tm1(CAG-Glra3*)Jcme} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Glra3 (114516)

  Glra3

  (MGI:95749)

• Mutation details: A region encompassing the 5' UTR through intron 2 was replaced with a construct containing CAG-loxP-stop-loxP-Glra3 cDNA (rat, long variant with a P185L substitution and containing exon 8a) and human HPRT promoter and exons 1 and 2. The CAG-Glra3 and Hprt sequences are expressed in opposite directions. The Glra3 sequence also has an HA tag. (J:207981)

Generation of the Hprt1^{tm1(CAG-Glra3*)Jcme} allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1273 strains or lines available

References

• Original: J:207981 Winkelmann A, et al., Changes in neural network homeostasis trigger neuropsychiatric symptoms. J Clin Invest. 2014 Feb 3;124(2):696-711
• All: 2 reference(s)