Gt(ROSA)26Sor^{tm3.1(CAG-SORL1)Tew}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3.1(CAG-SORL1)Tew}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3.1, Thomas E Willnow
• MGI ID: MGI:5586989
• Synonyms: Rosa26^Tg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3.1(CAG-SORL1)Tew} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213687
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm3.1(CAG-SORL1)Tew} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SORL1 (6653)

  Sorl1

  (MGI:1202296)

• Mutation details: The targeting construct inserted into the locus a wild-type human cDNA preceded by the cytomegalovirus early enhancer/chicken beta-actin promoter (CAG) element and a neomycin resistance cassette with a triple polyadenylation site flanked by loxP sites. Cre-mediated recombination removed the neomycin resistance cassette and allowed for expression of the human cDNA. Western blotting and ELISA indicate a fourfold elevation in SORL1 in the cortex and hippocampus of adult heterozygotes. (J:213687)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:213687 Caglayan S, et al., Lysosomal sorting of amyloid-beta by the SORLA receptor is impaired by a familial Alzheimer's disease mutation. Sci Transl Med. 2014 Feb 12;6(223):223ra20
• All: 5 reference(s)