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Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Feng Zhang
MGI ID: MGI:5583839
Synonyms: Cre-dependent Cas9, Gt(ROSA)26Sortm1(CAG-xstpx-cas9,-EGFP)Fezh, Rosa26-LSL-Cas9
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:213550
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Conditional ready, Endonuclease, Reporter)
Mutation:    Insertion
 
Mutation detailsThe targeting vector inserted into the locus was designed with (from 5' to 3') a 5' homology arm, a ubiquitously expressed CAG promoter, loxP-flanked 3xSV40 polyA stop cassette, a 3X-FLAG epitope tag, a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) flanked by two nuclear localization signals, a P2A ribosomal skip cleavage peptide sequence, an enhanced Green Fluorescent Protein (EGFP) gene, a woodchuck hepatitis virus post-transcriptional regulatory element, bovine growth hormone polyA, pPGK-Neo-pA positive selection cassette and a 3' homology arm. (J:213550)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 5 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  741 strains or lines available
References
Original:  J:213550 Platt RJ, et al., CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Oct 9;159(2):440-55
All:  53 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/16/2021
MGI 6.17
The Jackson Laboratory