Satb1^em1Bey
Endonuclease-mediated Allele Detail

Summary

• Symbol: Satb1^em1Bey
• Name: special AT-rich sequence binding protein 1; endonuclease-mediated mutation 1, Marc Beyer
• MGI ID: MGI:5575372
• Gene: Satb1 Location: Chr17:52043215-52140318 bp, - strand Genetic Position: Chr17, 26.81 cM
• Alliance: Satb1^em1Bey page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1 x C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: TALEN based technology was used to insert the following construct into the target gene. A loxP followed by lox2272 was inserted upstream of exon2 and its start cocon. This was followed by an FRT flanked neomycin/kanamycin construct in reverse orientation. This was followed in turn by an EGFP/Satb1 3'UTR, again in reverse orientation. Finally, a second loxP followed by lox2272 was inserted. Cre expression will invert the sequence between loxP sites allowing expression of EGFP. (J:206457)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Satb1 Mutation: 47 strains or lines available

References

• Original: J:206457 Sommer D, et al., Efficient genome engineering by targeted homologous recombination in mouse embryos using transcription activator-like effector nucleases. Nat Commun. 2014 Jan 13;5:3045
• All: 2 reference(s)