Jak2^tm2.1Kpf
Targeted Allele Detail

Summary

• Symbol: Jak2^tm2.1Kpf
• Name: Janus kinase 2; targeted mutation 2.1, Klaus Pfeffer
• MGI ID: MGI:5566794
• Synonyms: Jak2^FF
• Gene: Jak2 Location: Chr19:29229228-29290480 bp, + strand Genetic Position: Chr19, 23.73 cM
• Alliance: Jak2^tm2.1Kpf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207710
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 21 was replaced with one in which nucleotide substitutions result in the amino acid substitutions of phenyalanine for tyrosine at positions 1007 and 1008 in the activation loop. Cre-mediated recombination removed a floxed neomycin resistance cassette inserted upstream of exon 20. Western blot analysis confirmed normal protein levels. (J:207710)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Jak2 Mutation: 57 strains or lines available

References

• Original: J:207710 Keil E, et al., Important scaffold function of the Janus kinase 2 uncovered by a novel mouse model harboring a Jak2 activation-loop mutation. Blood. 2014 Jan 23;123(4):520-9
• All: 1 reference(s)