Kcnk16^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Kcnk16^{tm1a(EUCOMM)Hmgu}
• Name: potassium channel, subfamily K, member 16; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5565752
• Gene: Kcnk16 Location: Chr14:20311444-20319267 bp, - strand Genetic Position: Chr14, 11.49 cM, cytoband B
• IMPC: Kcnk16 gene page

Mutation origin

• Mutant Cell Lines: HEPD0968_4_A02, HEPD0968_4_A03, HEPD0968_4_B01, HEPD0968_4_B03, HEPD0968_4_B04, HEPD0968_4_C01, HEPD0968_4_C02, HEPD0968_4_C03, HEPD0968_4_C04, HEPD0968_4_D01, HEPD0968_4_E01, HEPD0968_4_E04, HEPD0968_4_F01, HEPD0968_4_F02, HEPD0968_4_F04, HEPD0968_4_G01, HEPD0968_4_G02, HEPD0968_4_G04, HEPD0968_4_H01, HEPD0968_4_H04
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 20313996 of Chromosome 14 upstream of the critical exon 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons 2-3 at position 20315779. The critical exons 2-3 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:344958)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kcnk16 Mutation: 10 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)