Slx4^tm1.2Jrou
Targeted Allele Detail

Summary

• Symbol: Slx4^tm1.2Jrou
• Name: SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae); targeted mutation 1.2, John Rouse
• MGI ID: MGI:5559219
• Gene: Slx4 Location: Chr16:3796969-3821634 bp, - strand Genetic Position: Chr16, 2.34 cM
• Alliance: Slx4^tm1.2Jrou page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204996
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 8. An FRT-flanked neomycin resistance cassette, IRES and splice donor with a 5' loxP site was inserted downstream of exon 9. Flp-mediated recombination removed the selection cassette and left exons 8 and 9 floxed. Western blot analysis confirmed the absence of protein expression in mosue embryonic extracts. (J:204996)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slx4 Mutation: 57 strains or lines available

References

• Original: J:204996 Castor D, et al., Cooperative control of holliday junction resolution and DNA repair by the SLX1 and MUS81-EME1 nucleases. Mol Cell. 2013 Oct 24;52(2):221-33
• All: 2 reference(s)