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Targeted Allele Detail
Symbol: Rock1tm1.2Itl
Name: Rho-associated coiled-coil containing protein kinase 1; targeted mutation 1.2, inGenious Targeting Laboratory
MGI ID: MGI:5556290
Gene: Rock1  Location: Chr18:10064401-10181792 bp, - strand  Genetic Position: Chr18, 4.91 cM, cytoband A2
Absence of autophagosomes in Rock1tm1.2Itl/Rock1tm1.2Itl starved hearts

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:207251
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsAn FRT- and loxP-flanked neomycin resistance cassette was inserted upstream of exon 5. An additional loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the neomycin resistance cassette. Cre-mediated recombination removed exon 5. Western blot analysis confirmed the absence of protein expression in the heart. (J:207251)
Generation of the Rock1tm1.1Itl and Rock1tm1.2Itl alleles
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rock1 Mutation:  8 strains or lines available
Original:  J:207251 Gurkar AU, et al., Identification of ROCK1 kinase as a critical regulator of Beclin1-mediated autophagy during metabolic stress. Nat Commun. 2013;4:2189
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.14
The Jackson Laboratory