Synmtm1.1(KOMP)Vlcg
Targeted Allele Detail
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Symbol: |
Synmtm1.1(KOMP)Vlcg |
Name: |
synemin, intermediate filament protein; targeted mutation 1.1, Velocigene |
MGI ID: |
MGI:5548847 |
Gene: |
Synm Location: Chr7:67379909-67409490 bp, - strand Genetic Position: Chr7, 37.12 cM
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Alliance: |
Synmtm1.1(KOMP)Vlcg page
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IMPC: |
Synm gene page |
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Mutant Cell Line: |
14923A-H9 |
Germline Transmission: |
Earliest citation of germline transmission:
J:204739
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Parent Cell Line: |
VGB6 (ES Cell)
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Strain of Origin: |
C57BL/6NTac
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Project Collection: |
KOMP-Regeneron
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Allele Type: |
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Targeted (Null/knockout, Reporter) |
Mutations: |
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Insertion, Intragenic deletion
Vector: ZEN-Ub1
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Mutation details: Cre-mediated excision of the parental Synmtm1(KOMP)Vlcg allele resulted in the removal of the neomycin selection cassette and exons 2 and 3, leaving behind the inserted lacZ reporter sequence. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml.
RT-PCR analysis of mRNA from skeletal muscle of homozygous mutant and control mice showed that the transcript is absent from the homozygotes but present in controls. Western blot analysis of protein supernatants prepared from extracts of tibialis anterior muscles confirmed the absence of encoded protein in homozygous mutant muscles.
(J:204739, J:224030)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:204739 International Knockout Mouse Consortium, MGI download of modified allele data from IKMC and creation of new knockout alleles. Database Download. 2014-2023; |
All: |
7 reference(s) |
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