Rab38^em5Rkuhn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rab38^em5Rkuhn
• Name: RAB38, member RAS oncogene family; endonuclease-mediated mutation 5, Ralf Kuhn
• MGI ID: MGI:5544456
• Synonyms: Rab38^IDG-Wt
• Gene: Rab38 Location: Chr7:88079481-88140780 bp, + strand Genetic Position: Chr7, 49.19 cM
• Alliance: Rab38^em5Rkuhn page

Mutation origin

• Strain of Origin: (FVB/NCrl x C57BL/6J-Rab38^cht)F1

Mutation description

Allele Type: Endonuclease-mediated Mutation: Nucleotide substitutions Mutation details: TALEN-targeted homologous recombination corrected the T to G nucleotide substitution in the chocolate mutation (Rab38^cht) by reconstituting the wild-type sequence encoding glycine at position 19 and thereby eliminating the exogenous SexAI restriction site (cht-GTT to wt-GGT). An identifying neutral nucleotide replacement in codon 18 (G to A) was used as a unique sequence identifier. Silent exchanges within the TALEN binding region remove an endogenous ApaLI site. This allele was identified in a line established from founder Rw2. (J:205086)

Generation of the Rab38^em5Rkuhn allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rab38 Mutation: 15 strains or lines available

References

• Original: J:205086 Wefers B, et al., Direct production of mouse disease models by embryo microinjection of TALENs and oligodeoxynucleotides. Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):3782-7
• All: 1 reference(s)