Igs1^{tm11(CAG-Bgeo,-Edn2)Nat}
Targeted Allele Detail

Summary

• Symbol: Igs1^{tm11(CAG-Bgeo,-Edn2)Nat}
• Name: intergenic site 1; targeted mutation 11, Jeremy Nathans
• MGI ID: MGI:5544083
• Synonyms: Z/Edn2
• Gene: Igs1 Location: unknown Genetic Position: Chr11, Syntenic
• Alliance: Igs1^{tm11(CAG-Bgeo,-Edn2)Nat} page

Retinal vascular defects in Igs1^{tm11(CAG-Bgeo,-Edn2)Nat}/Igs1⁺ Tg(Six3-cre)69Frty/0 mice

Show the 10 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:201133
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Igs1^{tm11(CAG-Bgeo,-Edn2)Nat} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Edn2 (MGI:95284)

• Mutation details: A site 5' of the Ubb gene was targeted with a construct composed of a CMV/beta-actin enhancer and promoter (CAG), a floxed beta-geo with three transcription termination sites and an Edn2 and IRES-GFP. Cre-mediated excision of the floxed beta-geo construct is required to achieve expression of the Edn2 and GFP cassette. (J:201133)

Generation of the Igs1^{tm11(CAG-Bgeo,-Edn2)Nat} allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igs1 Mutation: 10 strains or lines available

References

• Original: J:201133 Rattner A, et al., Endothelin-2 signaling in the neural retina promotes the endothelial tip cell state and inhibits angiogenesis. Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):E3830-E3839
• All: 2 reference(s)