Foxa2^tm2.1Heli
Targeted Allele Detail

Summary

• Symbol: Foxa2^tm2.1Heli
• Name: forkhead box A2; targeted mutation 2.1, Heiko Lickert
• MGI ID: MGI:5532711
• Synonyms: Foxa2^FVF, Foxa2^FVF-deltaNeo
• Gene: Foxa2 Location: Chr2:147884797-147888889 bp, - strand Genetic Position: Chr2, 73.38 cM
• Alliance: Foxa2^tm2.1Heli page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204475
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutation: Insertion Mutation details: A Venus gene was inserted in frame with the coding sequence in exon 3 resulting in a fusion product. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette inserted downstream of the reporter gene. Western blot analysis confirmed normal protein expression levels of the fusion protein in heterozygous mice. (J:204475)

Generation of the Foxa2^tm2.1Heli allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Foxa2 Mutation: 26 strains or lines available

References

• Original: J:204475 Burtscher I, et al., Foxa2-venus fusion reporter mouse line allows live-cell analysis of endoderm-derived organ formation. Genesis. 2013 Aug;51(8):596-604
• All: 5 reference(s)