Top1^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Top1^{tm1b(KOMP)Wtsi}
• Name: topoisomerase (DNA) I; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5509060
• Gene: Top1 Location: Chr2:160487901-160564684 bp, + strand Genetic Position: Chr2, 80.96 cM, cytoband H2-3
• Alliance: Top1^{tm1b(KOMP)Wtsi} page
• IMPC: Top1 gene page

Mutation origin

• Mutant Cell Line: EPD0033_2_C05
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 160511599 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site was inserted downstream of the targeted exon(s) at position 160512356. The critical exon(s) were thus flanked by loxP sites, and subsequent Cre-mediated excision deleted the critical sequence and the neomycin selection cassette yielding a knockout reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Top1 Mutation: 213 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)