Svep1^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Svep1^{tm1b(EUCOMM)Hmgu}
• Name: sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5509058
• Synonyms: Svep1^tm1b
• Gene: Svep1 Location: Chr4:58042796-58206596 bp, - strand Genetic Position: Chr4, 31.87 cM, cytoband B-C2
• Alliance: Svep1^{tm1b(EUCOMM)Hmgu} page
• IMPC: Svep1 gene page

Mutation origin

• Mutant Cell Line: HEPD0747_6_B06
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 58133064 of Chromosome 4 upstream of the critical exon (exon 8) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of exon 8 at position 58133064. Exon 8 is thus flanked by loxP sites, and subsequenct Cre-mediated excision deleted the critical sequence and neomycin selection cassette yielding a knockout reporter allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:157065, J:188991, J:255307)

Expression

In Mice Carrying this Mutation:	13 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Svep1 Mutation: 169 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 8 reference(s)