Gt(ROSA)26Sor^{tm1(Cacna1c*)Red}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Cacna1c*)Red}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Ricardo Dolmetsch
• MGI ID: MGI:5508369
• Synonyms: CaV1.2^TS, Rosa26^FMTYGR
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Cacna1c*)Red} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199433
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, No functional change)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(Cacna1c*)Red} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Cacna1c (24239)

  Cacna1c

  (MGI:103013)

  expresses a FLAG and Myc-tagged rat Cacna1c cDNA encoding channels with the T1036Y (dihydropyridine insensitive mutation) and the G406R (Timothy syndrome type 1 mutation, FMTYGR) mutations

• Gt(ROSA)26Sor^{tm1(Cacna1c*)Red} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Gt(ROSA)26Sor (MGI:104735)

• Mutation details: A targeting construct introduced into the locus a FLAG and Myc-tagged rat Cacna1c cDNA encoding channels with the T1036Y (dihydropyridine insensitive mutation) and the G406R (Timothy syndrome type 1 mutation, FMTYGR) mutations. A floxed stop cassette prevents expression of the inserted sequence until Cre-mediated recombination takes place. (J:199433)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:199433 Pasca SP, et al., Using iPSC-derived neurons to uncover cellular phenotypes associated with Timothy syndrome. Nat Med. 2011 Dec;17(12):1657-62
• All: 7 reference(s)