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Rorbtm1.1(cre)Hze
Targeted Allele Detail
Nomenclature
Symbol: Rorbtm1.1(cre)Hze
Name: RAR-related orphan receptor beta; targeted mutation 1.1, Hongkui Zeng
MGI ID: MGI:5507855
Synonyms: Rorb-IRES2-Cre, Rorb-IRES2-Cre-Delta, Rorb-IRES2-Cre-DeltaNeo/Hygro, Rorb-IRES2-Cre Rorb-IRES2-Cre-DeltaNeo/Hygro, Rorbm1(IRES2-Cre)Hze
Gene: Rorb  Location: Chr19:18907969-19088560 bp, - strand  Genetic Position: Chr19, 13.28 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:146821
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Mutation detailsThe targeting vector contained, from 5' to 3', a partial Rorb sequence from intron 9 (with an frt3), a partial Rorb exon 10 sequence including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence, a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. This construct was electroporated into G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL). The resulting Rorb-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice to remove PhiC31 transgene. (J:146821, J:220523)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Rorb     Summary of all recombinase alleles driven by Rorb.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Rorb Mutation:  25 strains or lines available
References
Original:  J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/16/2021
MGI 6.17
The Jackson Laboratory