Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey
Transgene Detail

Summary

• Symbol: Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey
• Name: transgene insertion 17.96, Claude-Agnes Reynaud
• MGI ID: MGI:5505386
• Synonyms: Lkappa(TG)11, Lk(TG)11
• Transgene: Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey Location: unknown
• Alliance: Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Igk-VOx1*/Igkj5/Igk-C*)17.96Crey expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Igk (MGI:96494)

• Mutation details: This transgene comprises two copies of a modified version of an immunoglobulin kappa light chain (Igk) construct containing the rearranged mouse 2-phenyl-oxazolone (phOx) specific VkOx1-Jk5 variable region, the mouse intronic Igk enhancer, the rat Igk constant region (Igk-C) and 8 kb of mouse 3'-flanking sequence including the 3' enhancer. The modification consists of the introduction of two eleven-TG dinucleotide tracts, one at the border of complementarity determining region 1 (CDR1) and framework region 2 (FR2) of the variable region and the second at the beginning of Igk-C. These insertions cause a frameshift that creates a stop codon at the beginning of CDR2, so the gene as passed through the germline is non-functional. It undergoes mutation in the B-cell lineage, at least in Peyer's patches. (J:199560)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:199560 Bertocci B, et al., Probing immunoglobulin gene hypermutation with microsatellites suggests a nonreplicative short patch DNA synthesis process. Immunity. 1998 Aug;9(2):257-65
• All: 1 reference(s)