Mmp25^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Mmp25^{tm1a(EUCOMM)Hmgu}
• Name: matrix metallopeptidase 25; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5497351
• Gene: Mmp25 Location: Chr17:23847289-23864006 bp, - strand Genetic Position: Chr17, 11.87 cM
• IMPC: Mmp25 gene page

Mutation origin

• Mutant Cell Lines: HEPD0886_7_A01, HEPD0886_7_A02, HEPD0886_7_A04, HEPD0886_7_A05, HEPD0886_7_A06, HEPD0886_7_A08, HEPD0886_7_A09, HEPD0886_7_A11, HEPD0886_7_B01, HEPD0886_7_B02, HEPD0886_7_B03, HEPD0886_7_B04, HEPD0886_7_B05, HEPD0886_7_B08, HEPD0886_7_B09, HEPD0886_7_B10, HEPD0886_7_B11, HEPD0886_7_B12, HEPD0886_7_C01, HEPD0886_7_C06, HEPD0886_7_C07, HEPD0886_7_C08, HEPD0886_7_C10, HEPD0886_7_C12, HEPD0886_7_D01, HEPD0886_7_D02, HEPD0886_7_D03, HEPD0886_7_D04, HEPD0886_7_D05, HEPD0886_7_D06, HEPD0886_7_D07, HEPD0886_7_D08, HEPD0886_7_D09, HEPD0886_7_D10, HEPD0886_7_D11, HEPD0886_7_D12, HEPD0886_7_E03, HEPD0886_7_E07, HEPD0886_7_E08, HEPD0886_7_E09, HEPD0886_7_E10, HEPD0886_7_E11, HEPD0886_7_E12, HEPD0886_7_F04, HEPD0886_7_F05, HEPD0886_7_F06, HEPD0886_7_F07, HEPD0886_7_F08, HEPD0886_7_F10, HEPD0886_7_F11, HEPD0886_7_F12, HEPD0886_7_G03, HEPD0886_7_G04, HEPD0886_7_G05, HEPD0886_7_G06, HEPD0886_7_G09, HEPD0886_7_G10, HEPD0886_7_G11, HEPD0886_7_H03, HEPD0886_7_H04, HEPD0886_7_H05, HEPD0886_7_H06, HEPD0886_7_H07, HEPD0886_7_H08, HEPD0886_7_H10, HEPD0886_7_H11, HEPD0886_7_H12
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 23862789 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 23863435. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mmp25 Mutation: 33 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)