Malat1^tm1.2Dlsp
Targeted Allele Detail

Summary

• Symbol: Malat1^tm1.2Dlsp
• Name: metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA); targeted mutation 1.2, David L Spector
• MGI ID: MGI:5496055
• Synonyms: Malat1 KO
• Gene: Malat1 Location: Chr19:5845718-5852700 bp, - strand Genetic Position: Chr19, 4.34 cM
• Alliance: Malat1^tm1.2Dlsp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196017
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neomycin resistance cassette with a 3' loxP site was inserted upstream of the coding sequence. An additional loxP site was inserted 1.5 kb downstream of the translation initiation site. Flp-mediated recombination removed the neomycin resistance cassette and left a portion of the coding sequence floxed. Cre-mediated recombination removed the floxed portion of the coding sequence. Northern blot analysis confirmed the absence of transcript expression in the brain and liver. (J:196017)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Malat1 Mutation: 8 strains or lines available

References

• Original: J:196017 Zhang B, et al., The lncRNA Malat1 is dispensable for mouse development but its transcription plays a cis-regulatory role in the adult. Cell Rep. 2012 Jul 26;2(1):111-23
• All: 6 reference(s)