Gt(ROSA)26Sor^{tm2(EIF1AX-bglA*)Dwi}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(EIF1AX-bglA*)Dwi}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Douglas J Winton
• MGI ID: MGI:5495167
• Synonyms: R26(SYNbglA)R
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(EIF1AX-bglA*)Dwi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:196052
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

Allele Type: Targeted (Conditional ready, Reporter) Mutation: Insertion Mutation details: The construct targeted to the locus contains a floxed neomycin resistance/STOP cassette, the human EIF1AX promoter, a Caldocellum saccharolyticum gene that encodes a beta-glucosidase (bglA) and a polyadenylation sequence. Cre-mediated recombination is required to achieve expression of the reporter except in the small intestine where background activity is detected. (J:196052)

Generation of the Gt(ROSA)26Sor^tm2(EIF1AX-bglA*)Dwi allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:196052 McCutcheon SC, et al., Characterization of a heat resistant beta-glucosidase as a new reporter in cells and mice. BMC Biol. 2010;8:89
• All: 1 reference(s)