Cd3e^tm1.1Geno
Targeted Allele Detail

Summary

• Symbol: Cd3e^tm1.1Geno
• Name: CD3 antigen, epsilon polypeptide; targeted mutation 1.1, Genoway
• MGI ID: MGI:5474199
• Synonyms: KI-PRS
• Gene: Cd3e Location: Chr9:44910038-44920925 bp, - strand Genetic Position: Chr9, 24.84 cM
• Alliance: Cd3e^tm1.1Geno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:192604
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An FRT flanked neo cassette was inserted into intron 6 (intron 4 in the paper) and two C to G mutations were inserted into exon 7 (coding exon 6; exon 5 in the paper) via homologous recombination. These mutations replace two central prolines in the proline-rich sequence with alanines (P163A, P166A). Interaction between the mutant protein and the SH3.1 domain of Nck1 is not seen in a pull down assay. Flp mediated recombination removed the neo cassette. (J:192604)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd3e Mutation: 47 strains or lines available

References

• Original: J:192604 Borroto A, et al., Nck Recruitment to the TCR Required for ZAP70 Activation during Thymic Development. J Immunol. 2013 Feb 1;190(3):1103-12
• All: 5 reference(s)