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Pvalbtm3.1(dreo)Hze
Targeted Allele Detail
Nomenclature
Symbol: Pvalbtm3.1(dreo)Hze
Name: parvalbumin; targeted mutation 3.1, Hongkui Zeng
MGI ID: MGI:5461318
Synonyms: Pvalb-2A-Dre, Pvalb-2A-DreO-Deltahygro, PvalbDre
Gene: Pvalb  Location: Chr15:78191114-78206400 bp, - strand  Genetic Position: Chr15, 36.93 cM, cytoband E
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:191266
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Mutation detailsES cells containing Pvalbtm1.1(cre)Aibs were retargeted to insert a viral 2A oligopeptide (T2A) that mediates ribosomal skipping and a mammalian-optimized D6 site-specific DNA recombinase (dreo recombinase) inserted immediately downstream of the parvalbumin translational STOP codon. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a D6 site-specific DNA recombinase optimized for mammalian cell expression (DreO recombinase), the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette (with an RNA spice donor-frt5 site-RNA spice acceptor in the hygromycin gene), and an attP site. PhiC31-mediated recombination removed the hygro cassette. (J:191266, J:219930)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Pvalb     Summary of all recombinase alleles driven by Pvalb. Comparative matrix view of recombinase activities
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pvalb Mutation:  24 strains or lines available
References
Original:  J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/23/2020
MGI 6.15
The Jackson Laboratory