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Targeted Allele Detail
Symbol: Pvalbtm3.1(dreo)Hze
Name: parvalbumin; targeted mutation 3.1, Hongkui Zeng
MGI ID: MGI:5461318
Synonyms: Pvalb-2A-Dre, Pvalb-2A-DreO-Deltahygro, PvalbDre
Gene: Pvalb  Location: Chr15:78075314-78090600 bp, - strand  Genetic Position: Chr15, 36.93 cM, cytoband E
Germline Transmission:  Earliest citation of germline transmission: J:191266
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
Mutation detailsES cells containing Pvalbtm1.1(cre)Aibs were retargeted to insert a viral 2A oligopeptide (T2A) that mediates ribosomal skipping and a mammalian-optimized D6 site-specific DNA recombinase (dreo recombinase) inserted immediately downstream of the parvalbumin translational STOP codon. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a D6 site-specific DNA recombinase optimized for mammalian cell expression (DreO recombinase), the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette (with an RNA spice donor-frt5 site-RNA spice acceptor in the hygromycin gene), and an attP site. PhiC31-mediated recombination removed the hygro cassette. (J:191266, J:219930)
 Tissue activity of this recombinase allele
Driver: Pvalb     Summary of all recombinase alleles driven by Pvalb. Comparative matrix view of recombinase activities

View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pvalb Mutation:  25 strains or lines available
Original:  J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory