Pvalb^{tm3.1(dreo)Hze}
Targeted Allele Detail

Summary

• Symbol: Pvalb^{tm3.1(dreo)Hze}
• Name: parvalbumin; targeted mutation 3.1, Hongkui Zeng
• MGI ID: MGI:5461318
• Synonyms: Pvalb-2A-Dre, Pvalb-2A-DreO-Deltahygro, Pvalb^Dre
• Gene: Pvalb Location: Chr15:78075314-78090600 bp, - strand Genetic Position: Chr15, 36.93 cM, cytoband E
• Alliance: Pvalb^{tm3.1(dreo)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:191266
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Pvalb^{tm3.1(dreo)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pvalb (MGI:97821)

• Mutation details: ES cells containing Pvalb^{tm1.1(cre)Aibs} were retargeted to insert a viral 2A oligopeptide (T2A) that mediates ribosomal skipping and a mammalian-optimized D6 site-specific DNA recombinase (dreo recombinase) inserted immediately downstream of the parvalbumin translational STOP codon. Correctly targeted ES cells had (from 5' to 3') a three amino acid linker sequence, an frt3 site within Pvalb intron 3, a partial Pvalb exon 4 sequence up to (but not including) the endogenous stop codon, a T2A sequence that is in-frame with the Pvalb coding sequence, a D6 site-specific DNA recombinase optimized for mammalian cell expression (DreO recombinase), the Pvalb 3' UTR sequence from exon 4, an attB site, a PGK-hygromycin-SV40polyA cassette (with an RNA spice donor-frt5 site-RNA spice acceptor in the hygromycin gene), and an attP site. PhiC31-mediated recombination removed the hygro cassette. (J:191266, J:219930)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pvalb (mouse) Summary of all recombinase alleles driven by Pvalb. Alliance mouse Pvalb gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pvalb Mutation: 29 strains or lines available

References

• Original: J:219930 Madisen L, et al., Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance. Neuron. 2015 Mar 4;85(5):942-58
• All: 7 reference(s)