Pdx1^tm2Mgn
Targeted Allele Detail

Summary

• Symbol: Pdx1^tm2Mgn
• Name: pancreatic and duodenal homeobox 1; targeted mutation 2, Mark A Magnuson
• MGI ID: MGI:5438568
• Synonyms: Pdx1^CFP
• Gene: Pdx1 Location: Chr5:147206907-147212658 bp, + strand Genetic Position: Chr5, 86.84 cM
• Alliance: Pdx1^tm2Mgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187872
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

Allele Type: Targeted (Null/knockout, RMCE-ready, Reporter) Mutations: Insertion, Intragenic deletion Mutation details: Using reccombinase mediated cassette exchange (RMCE) using Pdx1^tm1Mgn, a FRT-flanked hygro cassette was inserted upstream of Area IV and nuclear localized CFP was inserted into exon 1 at the translation initiation site replacing part of the exon. Flp-mediated recombination removed the hygro cassette. A lox66/71 site remains upstream of Area IV and a lox2272 site downstream of CFP. (J:187872)

Overall scheme for generating the Pdx1^tm1Mgn and Pdx1^tm2Mgn alleles

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pdx1 Mutation: 35 strains or lines available

References

• Original: J:187872 Potter LA, et al., A recombinase-mediated cassette exchange-derived cyan fluorescent protein reporter allele for Pdx1. Genesis. 2012 Apr;50(4):384-92
• All: 3 reference(s)