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Targeted Allele Detail
Symbol: Pdx1tm2Mgn
Name: pancreatic and duodenal homeobox 1; targeted mutation 2, Mark A Magnuson
MGI ID: MGI:5438568
Synonyms: Pdx1CFP
Gene: Pdx1  Location: Chr5:147206907-147212658 bp, + strand  Genetic Position: Chr5, 86.84 cM
Germline Transmission:  Earliest citation of germline transmission: J:187872
Parent Cell Line:  TL1/TL-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Null/knockout, RMCE-ready, Reporter)
Mutations:    Insertion, Intragenic deletion
Mutation detailsUsing reccombinase mediated cassette exchange (RMCE) using Pdx1tm1Mgn, a FRT-flanked hygro cassette was inserted upstream of Area IV and nuclear localized CFP was inserted into exon 1 at the translation initiation site replacing part of the exon. Flp-mediated recombination removed the hygro cassette. A lox66/71 site remains upstream of Area IV and a lox2272 site downstream of CFP. (J:187872)
Overall scheme for generating the Pdx1tm1Mgn and Pdx1tm2Mgn alleles
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pdx1 Mutation:  27 strains or lines available
Original:  J:187872 Potter LA, et al., A recombinase-mediated cassette exchange-derived cyan fluorescent protein reporter allele for Pdx1. Genesis. 2012 Apr;50(4):384-92
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory