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Pdx1tm2Mgn
Targeted Allele Detail
Nomenclature
Symbol: Pdx1tm2Mgn
Name: pancreatic and duodenal homeobox 1; targeted mutation 2, Mark A Magnuson
MGI ID: MGI:5438568
Synonyms: Pdx1CFP
Gene: Pdx1  Location: Chr5:147270131-147275149 bp, + strand  Genetic Position: Chr5, 86.84 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:187872
Parent Cell Line:  TL1/TL-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Null/knockout, RMCE-ready, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsUsing reccombinase mediated cassette exchange (RMCE) using Pdx1tm1Mgn, a FRT-flanked hygro cassette was inserted upstream of Area IV and nuclear localized CFP was inserted into exon 1 at the translation initiation site replacing part of the exon. Flp-mediated recombination removed the hygro cassette. A lox66/71 site remains upstream of Area IV and a lox2272 site downstream of CFP. (J:187872)
Overall scheme for generating the Pdx1tm1Mgn and Pdx1tm2Mgn alleles
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pdx1 Mutation:  22 strains or lines available
References
Original:  J:187872 Potter LA, et al., A recombinase-mediated cassette exchange-derived cyan fluorescent protein reporter allele for Pdx1. Genesis. 2012 Apr;50(4):384-92
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/13/2019
MGI 6.14
The Jackson Laboratory