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| Caption | Overall scheme for generating the Pdx1tm1Mgn and Pdx1tm2Mgn alleles. (a) Diagram of the wild-type Pdx1 locus, gene targeting vector, Pdx1tm1Mgn (Pdx1LCA) allele, exchange vector, Pdx1CFP1+HygroR allele, and Pdx1tm2Mgn (Pdx1CFP) allele. Homologous recombination resulted in replacement of an 8.6 kb region of the Pdx1 locus containing exon 1 and Areas I-IV with both a Pgk-driven puromycin resistance-D-thymidine kinase fusion gene (puDTK) and an EM7-driven kanamycin resistance (KanR) gene flanked by lox71 (open triangle) and lox2272 (black triangle) sites. The exchange vector contained lox66 (red triangle) and lox2272 sites flanking a nuclear-localized CFP sequence followed by a rabbit beta-globin polyadenylation sequence. A Pgk-driven hygromycin resistance (HygroR) cassette flanked by FRT sites (open circles) was used as a positive selectable marker during recombinase-mediated cassette exchange (RMCE). Mice containing the Pdx1CFP1+HygroR allele were bred with FLPe-expressing transgenic mice to remove the FRT-flanked HygroR cassette. Restriction sites: XmaI (X) and SphI (S). Primer locations: p1, p2, p3, and p4. | ||||||
| Copyright | This image is from Potter LA, Genesis 2012 Apr;50(4):384-92, and is displayed with the permission of Wiley-Blackwell, who owns the Copyright. J:187872 | ||||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/23/2024 MGI 6.23 |
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