Gja10^{tm2(Hbegf/EGFP)Kwi}
Targeted Allele Detail

Summary

• Symbol: Gja10^{tm2(Hbegf/EGFP)Kwi}
• Name: gap junction protein, alpha 10; targeted mutation 2, Klaus Willecke
• MGI ID: MGI:5433359
• Synonyms: Cx57^DTR, Cx57-DTRfrtCre
• Gene: Gja10 Location: Chr4:32596960-32602760 bp, - strand Genetic Position: Chr4, 14.25 cM
• Alliance: Gja10^{tm2(Hbegf/EGFP)Kwi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186624
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Gja10^{tm2(Hbegf/EGFP)Kwi} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Hbegf

• Mutation details: Homologous recombination replaced most of exon 2 with an FRT-flanked sequence encoding an HBEGF/EGFP (DTR/EGFP) fusion protein followed by cre recombinase with a nuclear localization signal. A PGK-neo selection cassette was inserted into intron 2. The diphtheria toxin receptor (HBEGF or DTR) is expressed from the Gja10 promoter and can be removed by flp-mediated recombination, leaving cre recombinase under control of the promoter. (J:167075, J:186624)

Generation of Gja10^{tm2(Hbegf/EGFP)Kwi}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gja10 Mutation: 22 strains or lines available

References

• Original: J:186624 Sonntag S, et al., Ablation of retinal horizontal cells from adult mice leads to rod degeneration and remodeling in the outer retina. J Neurosci. 2012 Aug 1;32(31):10713-24
• All: 5 reference(s)