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Gt(ROSA)26Sortm3(CAG-flpo/ERT2)Alj
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm3(CAG-flpo/ERT2)Alj
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Alexandra L Joyner
MGI ID: MGI:5432216
Synonyms: Gt(ROSA)26Sortm3(ACTB-FLPo/ERT2)Alj, R26FlpoER
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:186310
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Inducible, Recombinase)
Inducer:    tamoxifen
Mutation:    Insertion
 
Mutation detailsThe sequence encoding a FLPo/ERT2 fusion protein (optimized FLP recombinase fused to the tamoxifen-responsive mutated human estrogen receptor ligand-binding domain), SV40 polyadenylation site and a loxP-flanked PGK-neo cassette was cloned downstream of the human CMV enhancer/chicken ACTB promoter (CAG). This construct was targeted to the Gt(ROSA)26Sor locus in embryonic stem cells. (J:186310)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: CAG     Summary of all recombinase alleles driven by CAG.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  741 strains or lines available
Notes
ES cell line = CY2.3 (129S6/SvEvTac-derived: gift of Rockefeller Gene Targeting Core)
References
Original:  J:186310 Lao Z, et al., MASTR: a technique for mosaic mutant analysis with spatial and temporal control of recombination using conditional floxed alleles in mice. Cell Rep. 2012 Aug 30;2(2):386-96
All:  13 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory