Mir341^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir341^tm1Wtsi
• Name: microRNA 341; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5320113
• Synonyms: Mir341^{tm1(mirKO)Wtsi}
• Gene: Mir341 Location: Chr12:109577934-109578029 bp, + strand Genetic Position: Chr12, 60.43 cM

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_8B1, mirKO_ES_PuDtk_8B2, mirKO_ES_PuDtk_8B3
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: PGK_EM7_PuDtk_bGHpA
• Mutation details: The microRNA gene was replaced via homologous recombination by a LoxP-F3-PGK-EM7-Purotk-bpA-LoxP-FRT targeting vector with 5.5kb (5') and 3.3kb (3') arms of homology. Briefly, this is a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters and flanked by LoxP and F3/FRT sites. The deletion starts at position 109577921 and ends at position 109578040 of Chromosome 12 (Build GRCm39). (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir341 Mutation: 2 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)