Mir499^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir499^tm1Wtsi
• Name: microRNA 499; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5320065
• Synonyms: Mir499^{tm1(mirKO)Wtsi}
• Gene: Mir499 Location: Chr2:155464800-155464878 bp, + strand Genetic Position: Chr2, 77.26 cM

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_12H1, mirKO_ES_PuDtk_12H2, mirKO_ES_PuDtk_12H3, mirKO_ES_PuDtk_12H4
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: PGK_EM7_PuDtk_bGHpA
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 3424 starting at position 155330353 and ending at position 155333777 of Chromosome 2 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir499 Mutation: 1 strain or line available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)