Gt(ROSA)26Sor^{tm8(Hoxd11)Amc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm8(Hoxd11)Amc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 8, Andrew P McMahon
• MGI ID: MGI:5315142
• Synonyms: R26Hoxd11
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm8(Hoxd11)Amc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:137524
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129 and C57BL/6 and CBA and Swiss Webster

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm8(Hoxd11)Amc} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Hoxd11 (MGI:96203)

• Mutation details: A cassette containing Hoxd11-IRES2-nlslacZ was inserted into the Gt(ROSA)26Sor locus via homologous recombination in YFP3.1 ES cells. The inserted cassette has a floxed PGK-neo STOP sequence preceding it, preventing Hoxd11 or lacZ transcription unless cre-mediated excision of the STOP has taken place. Targeted ES cells were injected into C57BL/6J blastocysts. This line was maintained on a C57BL/6J background. (J:137524)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

Notes

ES cell line = YFP3.1, a male ES cell line carrying Tg(CAG-cre/Esr1*)5Amc and is compound heterozgous at Gt(ROSA)26Sor: Gt(ROSA)26Sor^tm1Sor and Gt(ROSA)26Sor^tm1(EYFP)Cos.

References

• Original: J:137524 Mugford JW, et al., Hoxd11 specifies a program of metanephric kidney development within the intermediate mesoderm of the mouse embryo. Dev Biol. 2008 Jul 15;319(2):396-405
• All: 1 reference(s)