Pcdha^{tm2.1(DTA)Tman}
Targeted Allele Detail

Summary

• Symbol: Pcdha^{tm2.1(DTA)Tman}
• Name: protocadherin alpha cluster; targeted mutation 2.1, Tom Maniatis
• MGI ID: MGI:5297097
• Synonyms: HS7
• Gene: Pcdha Location: unknown Genetic Position: Chr18, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:177466
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Hypomorph, Inserted expressed sequence, Modified regulatory region)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The diphtheria toxin A gene and a floxed hygro cassette replaced the HS7 (a neuron-specific transcriptional enhancer). A floxed selection cassette was removed by cre-mediated recombination. RT-PCR analysis on whole brain and cerebellum confirmed moderate reduction in transcript expression of all genes in the cluster. However, expression is unaffected in the cortex, hippocampus, and olfactory bulb. (J:177466)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pcdha Mutation: 52 strains or lines available

References

• Original: J:177466 Kehayova P, et al., Regulatory elements required for the activation and repression of the protocadherin-{alpha} gene cluster. Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):17195-200
• All: 1 reference(s)