Gt(ROSA)26Sor^{tm1(tTA,CMV*1-Rheb,-EGFP)Gtm}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(tTA,CMV*1-Rheb,-EGFP)Gtm}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, David H Gutmann
• MGI ID: MGI:5292522
• Synonyms: LSL-Rheb
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(tTA,CMV*1-Rheb,-EGFP)Gtm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:176586
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter, Transactivator)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(tTA,CMV*1-Rheb,-EGFP)Gtm} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Rheb (26954)

  Rheb	(MGI:97912)

• Mutation details: A construct, which contained a floxed neo cassette, regulated transcriptional activator (tTA), insulator, the tTA responsive CMV*1 promoter, rabbit beta-globin second intron, an HA-tagged rat Rheb gene, and IRES-eEGFP, was inserted into the locus. (J:176586)

Generation of the Gt(ROSA)26Sor^{tm1(tTA,CMV*1-Rheb,-EGFP)Gtm} allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:176586 Banerjee S, et al., Neurofibromatosis-1 regulates mTOR-mediated astrocyte growth and glioma formation in a TSC/Rheb-independent manner. Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15996-6001
• All: 2 reference(s)