Etv6^{tm1.1(RUNX1,hsb5)Lvdw}
Targeted Allele Detail

Summary

• Symbol: Etv6^{tm1.1(RUNX1,hsb5)Lvdw}
• Name: ets variant 6; targeted mutation 1.1, Louise van der Weyden
• MGI ID: MGI:5284820
• Synonyms: Etv6^RUNX1
• Gene: Etv6 Location: Chr6:134012663-134247121 bp, + strand Genetic Position: Chr6, 64.58 cM, cytoband G2
• Alliance: Etv6^{tm1.1(RUNX1,hsb5)Lvdw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:174866
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Mutation details: A construct, which contains a lox66, exons 1 through 6 of human RUNX1 (hAML1), IRES, hyperactive variant of sleeping beauty (hsb5), polyadenylation sequence, FRT-flanked puro cassette, and lox71 site, was inserted into intron 5. Mice express a Etv6/RUNX1 fusion to replicate the TEL-AML1 translocation associated with B-cell precursor acute lymphoblastic leukemia (BCP-ALL) and hsb5. Flp-mediated recombination removed the puro cassette. Embryonic lethality indicates that no functional endogenous protein is produced despite the production of the endogenous transcript. (J:174866)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Etv6 Mutation: 142 strains or lines available

References

• Original: J:174866 van der Weyden L, et al., Modeling the evolution of ETV6-RUNX1-induced B-cell precursor acute lymphoblastic leukemia in mice. Blood. 2011 Jul 28;118(4):1041-51
• All: 2 reference(s)