Mdm4^tm1Zmy
Targeted Allele Detail

Summary

• Symbol: Mdm4^tm1Zmy
• Name: transformed mouse 3T3 cell double minute 4; targeted mutation 1, Zhi-Min Yuan
• MGI ID: MGI:5141749
• Synonyms: Mdmx^C462A
• Gene: Mdm4 Location: Chr1:132913843-132958325 bp, - strand Genetic Position: Chr1, 57.75 cM, cytoband G-G
• Alliance: Mdm4^tm1Zmy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:174359
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 12 was replaced with a mutated version where cysteine codon 462 (TGT) was changed to alanine (GCT) (p.C462A) and a neomycin resistance gene cassette was inserted into intron 11. RT-PCR analysis confirmed the expression of the mutant transcript. This mutation interferes with binding to Mdm2. Immunohistochemical analysis indicates protein expression is increased in homozygous embryos compared to heterozygous or wild-type mice. (J:174359)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mdm4 Mutation: 191 strains or lines available

References

• Original: J:174359 Huang L, et al., The p53 inhibitors MDM2/MDMX complex is required for control of p53 activity in vivo. Proc Natl Acad Sci U S A. 2011 Jul 19;108(29):12001-6
• All: 2 reference(s)