Mirc1^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc1^tm1Wtsi
• Name: microRNA cluster 1, including Mir17 through Mir92-1; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5050730
• Synonyms: Mir17/Mir92a-1 cluster 14N2, Mir17^{tm1(mirKO)Wtsi}
• Gene: Mirc1 Location: Chr14:115281103-115281938 bp Genetic Position: Chr14, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_4F7, mirKO_ES_PuDtk_4F8, mirKO_ES_PuDtk_4F9, mirKO_ES_PuDtk_4F10, mirKO_ES_PuDtk_4F11
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: The microRNA cluster including Mir17, Mir18, Mir19a, Mir20a, Mir19b-1, and Mir92-1 was replaced via homologous recombination with a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters. The inserted selection cassette was flanked by LoxP and F3/FRT sites. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc1 Mutation: 6 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)