Tg(tetO-Fasl)BDepa
Transgene Detail

Summary

• Symbol: Tg(tetO-Fasl)BDepa
• Name: transgene insertion B, Monique De Paepe
• MGI ID: MGI:5050406
• Synonyms: (tetOp)7-FasL transgenic line B, TetOp-FasL transgenic line B
• Transgene: Tg(tetO-Fasl)BDepa Location: unknown
• Alliance: Tg(tetO-Fasl)BDepa page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-Fasl)BDepa expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Fasl (MGI:99255)

• Mutation details: The (tetO)7-FasL transgene was designed with a Tet response element (TRE) upstream of the 943 bp murine Fas ligand (FasL) coding sequence, all followed by a bovine growth hormone intron and polyA sequence. The Tet response element (TRE) contains seven copies of the 42-bp tet operator sequence (tetO) just upstream of a minimal CMV promoter. Five founder lines (A-E) were established. Founder mice from line B were found with ~6 copies of the transgene and high levels of doxycycline-induced FasL mRNA up-regulation. (J:137382)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:169681 De Paepe ME, et al., Alveolar epithelial cell therapy with human cord blood-derived hematopoietic progenitor cells. Am J Pathol. 2011 Mar;178(3):1329-39
• All: 3 reference(s)