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Targeted Allele Detail
Symbol: Gnas/Nespastm2.1Jop
Name: GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus; targeted mutation 1.1, Jo Peters
MGI ID: MGI:4950066
Synonyms: Nespas-Tex1
Gene: Gnas  Location: Chr2:174284320-174346744 bp, + strand  Genetic Position: Chr2, 97.89 cM, cytoband E1-H3
Germline Transmission:  Earliest citation of germline transmission: J:171195
Parent Cell Line:  CJ7 (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ Kitl+
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
Mutation detailsA cassette containing a polyadenylation sequence in the same orientation as Nespas and a self-excising neo cassette was inserted into exon 1. Cre-mediated recombination removed the neo cassette. The absence of Nespas transcript expression was confirmed by RT-qPCR on newborn brain extracts with paternal inheritance of the allele. De-repression of Gnas was confirmed by RT-qPCR. (J:171195)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gnas Mutation:  21 strains or lines available
Original:  J:171195 Williamson CM, et al., Uncoupling antisense-mediated silencing and DNA methylation in the imprinted gnas cluster. PLoS Genet. 2011 Mar;7(3):e1001347
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.08
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