About   Help   FAQ
Gnas/Nespastm2.1Jop
Targeted Allele Detail
Nomenclature
Symbol: Gnas/Nespastm2.1Jop
Name: GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus; targeted mutation 1.1, Jo Peters
MGI ID: MGI:4950066
Synonyms: Nespas-Tex1
Gene: Gnas  Location: Chr2:174284320-174346744 bp, + strand  Genetic Position: Chr2, 97.89 cM, cytoband E1-H3
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:171195
Parent Cell Line:  CJ7 (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsA cassette containing a polyadenylation sequence in the same orientation as Nespas and a self-excising neo cassette was inserted into exon 1. Cre-mediated recombination removed the neo cassette. The absence of Nespas transcript expression was confirmed by RT-qPCR on newborn brain extracts with paternal inheritance of the allele. De-repression of Gnas was confirmed by RT-qPCR. (J:171195)
Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gnas Mutation:  21 strains or lines available
References
Original:  J:171195 Williamson CM, et al., Uncoupling antisense-mediated silencing and DNA methylation in the imprinted gnas cluster. PLoS Genet. 2011 Mar;7(3):e1001347
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
03/14/2017
MGI 6.08
The Jackson Laboratory