Dock4^{tm1(NCOM)Mfgc}
Targeted Allele Detail

Summary

• Symbol: Dock4^{tm1(NCOM)Mfgc}
• Name: dedicator of cytokinesis 4; targeted mutation 1, Mammalian Functional Genomics Centre
• MGI ID: MGI:4880372
• Synonyms: Dock4^-
• Gene: Dock4 Location: Chr12:40495956-40896873 bp, + strand Genetic Position: Chr12, 18.06 cM
• Alliance: Dock4^{tm1(NCOM)Mfgc} page
• IMPC: Dock4 gene page

Dock4^{tm1(NCOM)Mfgc}/Dock4⁺ embryos show an increase in the frequency of blood vessels with small lumen width and a decrease in the frequency of large lumen width in the brain

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: N01793P1_C_109W_G1, N01793P1_C_109W_G2
• Germline Transmission: Earliest citation of germline transmission: J:224385
• Parent Cell Line: C2 (Tcp) (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: NorCOMM

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Vector: L1L2_GOHANU Mutation details: The insertion of NorCOMM cassette pGOHANU created a deletion of size 8521bp between positions 40673346-40681867 of Chromosome 12 (Build GRCm39). This deletion results in the removal of functionally critical exons 3-5. The cassette is composed of an F3 site followed by a universal inverse PCR primer site, splice acceptor IRES-lacZ sequence and a loxP site. This first loxP site is followed by deltaTK and neomycin under the control of the human beta-actin promoter / deltaTK / neomycin phosphotransferase (neoR) gene / SV40 polyA and a second loxP site. After the second loxP site is a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165964, J:224385)

Generation of the Dock4^tm1(NCOM)Mfgc allele

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dock4 Mutation: 108 strains or lines available

References

• Original: J:165964 Mammalian Functional Genomics Centre, Alleles produced for the NorCOMM project by the Mammalian Functional Genomics Centre (Mfgc), University of Manitoba. MGI Direct Data Submission. 2010
• All: 2 reference(s)