Gm8325^tm1(KOMP)Mbp
Targeted Allele Detail

Summary

• Symbol: Gm8325^tm1(KOMP)Mbp
• Name: predicted pseudogene 8325; targeted mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:4867984
• Gene: Gm8325 Location: Chr3:60784446-60785008 bp, + strand Genetic Position: Chr3, 29.17 cM
• IMPC: Gm8325 gene page

Mutation origin

• Mutant Cell Lines: DEPD00547_1_A04, DEPD00547_1_B04, DEPD00547_1_C01, DEPD00547_1_D03, DEPD00547_1_E03, DEPD00547_1_F02, DEPD00547_1_G04, DEPD00547_1_H02
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 511 starting at position 60784553 and ending at position 60785064 of Chromosome 3 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Gm8325 Mutation: 2 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 1 reference(s)