Taf3tm1a(KOMP)Mbp
Targeted Allele Detail
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Symbol: |
Taf3tm1a(KOMP)Mbp |
Name: |
TATA-box binding protein associated factor 3; targeted mutation 1a, Mouse Biology Program, UC Davis |
MGI ID: |
MGI:4843751 |
Gene: |
Taf3 Location: Chr2:9919363-10053407 bp, - strand Genetic Position: Chr2, 6.74 cM, cytoband A1
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Alliance: |
Taf3tm1a(KOMP)Mbp page
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IMPC: |
Taf3 gene page |
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Mutant Cell Lines: |
DEPD00008_5_A07, DEPD00008_5_A09, DEPD00008_5_A12, DEPD00008_5_B09, DEPD00008_5_B11, DEPD00008_5_B12, DEPD00008_5_C07, DEPD00008_5_E08, DEPD00008_5_E09, DEPD00008_5_F08, DEPD00008_5_F12, DEPD00008_5_G11, DEPD00008_5_H07, DEPD00008_5_H08, DEPD00008_5_H11, DEPD00008_5_H12 |
Germline Transmission: |
Earliest citation of germline transmission:
J:204812
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Parent Cell Line: |
JM8A1.N3 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 9955742 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 9958114. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157064)
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Original: |
J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010; |
All: |
2 reference(s) |
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