Katnal2^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Katnal2^{tm1a(EUCOMM)Wtsi}
• Name: katanin p60 subunit A-like 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4842422
• Synonyms: Katnal2^KO
• Gene: Katnal2 Location: Chr18:77064844-77135004 bp, - strand Genetic Position: Chr18, 52.02 cM, cytoband E3
• Alliance: Katnal2^{tm1a(EUCOMM)Wtsi} page
• IMPC: Katnal2 gene page

Spermiation and spermatid defects in Katnal2^m1Anu/Katnal2^{tm1a(EUCOMM)Wtsi} mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: EPD0656_4_A03, EPD0656_4_A04, EPD0656_4_B03, EPD0656_4_B04, EPD0656_4_C03, EPD0656_4_D02, EPD0656_4_D04, EPD0656_4_E02, EPD0656_4_E03, EPD0656_4_F01, EPD0656_4_F02, EPD0656_4_F03, EPD0656_4_G01, EPD0656_4_H02, EPD0656_4_H04
• Germline Transmission: Earliest citation of germline transmission: J:250115
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

Allele Type: Targeted (Conditional ready, Null/knockout, Reporter) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 77104991 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 77105793. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534, J:250115)

Schematic of the Katnal2^tm1a(EUCOMM)Wtsi knockout first, conditional-ready allele

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Katnal2 Mutation: 199 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)