Vipas39tm1a(KOMP)Mbp
Targeted Allele Detail
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Symbol: |
Vipas39tm1a(KOMP)Mbp |
Name: |
VPS33B interacting protein, apical-basolateral polarity regulator, spe-39 homolog; targeted mutation 1a, Mouse Biology Program, UC Davis |
MGI ID: |
MGI:4840775 |
Gene: |
Vipas39 Location: Chr12:87285642-87313030 bp, - strand Genetic Position: Chr12, 41.44 cM
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Alliance: |
Vipas39tm1a(KOMP)Mbp page
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IMPC: |
Vipas39 gene page |
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Mutant Cell Lines: |
DEPD00535_2_A09, DEPD00535_2_A10, DEPD00535_2_A11, DEPD00535_2_A12, DEPD00535_2_B09, DEPD00535_2_B10, DEPD00535_2_B11, DEPD00535_2_B12, DEPD00535_2_C11, DEPD00535_2_D09, DEPD00535_2_D10, DEPD00535_2_D12, DEPD00535_2_E10, DEPD00535_2_E12, DEPD00535_2_F09, DEPD00535_2_F11, DEPD00535_2_F12, DEPD00535_2_G11, DEPD00535_2_H09, DEPD00535_2_H10, DEPD00535_2_H12 |
Germline Transmission: |
Earliest citation of germline transmission:
J:236095
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
KOMP-CSD
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 87298249 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 87299141. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157064)
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Original: |
J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010; |
All: |
2 reference(s) |
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