Gt(ROSA)26Sor^{tm1.1(rtTA*M2,tetO-GFP)Rsp}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(rtTA*M2,tetO-GFP)Rsp}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Rolf Sprengel
• MGI ID: MGI:4839477
• Synonyms: R26, R26^t1delta, ROSA26-iM2-GFP, ROSA26-iM2-tetO-GFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(rtTA*M2,tetO-GFP)Rsp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165820
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Inducible, Reporter, Transactivator) Inducer: doxycycline/tetracycline Mutation: Insertion Mutation details: The construct containing the following components (5' to 3') was inserted into the locus: a splice acceptor sequence, improved transactivator (rtTA*M2) with a poladenylation sequence, floxed neo/ura selection cassette, a second polyadenylation site, and tetO driven GFP with a human growth hormone polyadenylation sequence. Cre-mediated recombination removed the neo/ura cassette. (J:165820)

Generation of the Gt(ROSA)26Sor^tm1.1(rtTA*M2,tetO-GFP)Rsp allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1060 strains or lines available

References

• Original: J:165820 Wortge S, et al., Tetracycline-controlled transgene activation using the ROSA26-iM2-GFP knock-in mouse strain permits GFP monitoring of DOX-regulated transgene-expression. BMC Dev Biol. 2010;10:95
• All: 2 reference(s)