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| Caption | Generation of the Gt(ROSA)26Sortm1.1(rtTA*M2,tetO-GFP)Rsp allele. The gene targeted R26t1delta allele controls the expression of DOX-inducible transcription from the endogenous promoter of the Gt(ROSA)26Sor locus. A) Gt(ROSA)26Sor and Gt(ROSA)26t1 (R26Rt1) loci. Exons are indicated as grey boxes; light grey show alternative spliced exons. Genetic elements inserted at the first XbaI site in intron1 of Gt(ROSA)26Sor are given in coloured boxes: first (yellow), the adenovirus major late splice acceptor sequence followed by the small intron of the adenoviral tripartite leader and the iM2 coding regionterminated by the human growth hormone polyadenylation signal (hgh_pA); second (red), a loxP site (black triangles) flanked neomycin/uracil selection cassette terminated by hgh_pA; third (green), the PtetO5-GFP gene module terminated by SV40_pA. Diagnostic restriction sites are indicated (E = EcoRV, Xh = XhoI, X = XbaI, P = PacI, A = AscI). The transparent green bar represents the chromosomal region covered by the targeting vector. Red-framed black boxes below R26Rt1 mark positions of Southern blot probes that detect restriction fragments given as red lines in kilo bases (kb). | ||||
| Copyright | This image is from Wortge S, BMC Dev Biol 2010;10():95, an open-access article, licensee BioMed Central Ltd. J:165820 | ||||
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Associated Alleles |
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 12/30/2025 MGI 6.24 |
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