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Igs2tm1(ACTB-EGFP,-tdTomato)Luo
Targeted Allele Detail
Nomenclature
Symbol: Igs2tm1(ACTB-EGFP,-tdTomato)Luo
Name: intergenic site 2; targeted mutation 1, Liqun Luo
MGI ID: MGI:4837736
Synonyms: GT: GFPN-termtdTC-term, Iis2tm1(ACTB-EGFP,-tdTomato)Luo, MADM-11GT, MADM-11-GT, Tg(ACTB-EGFP,-tdTomato)11Luo
Gene: Igs2  Location: unknown  Genetic Position: Chr11, Syntenic
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:166047
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsThe GT MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire GT MADM construct was inserted into the "Hipp11" locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). (J:166047)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 7 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Igs2 Mutation:  36 strains or lines available
References
Original:  J:166047 Hippenmeyer S, et al., Genetic mosaic dissection of lis1 and ndel1 in neuronal migration. Neuron. 2010 Nov 18;68(4):695-709
All:  24 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/06/2021
MGI 6.16
The Jackson Laboratory