Tg(Adipoq-cre)1Evdr
Transgene Detail

Summary

• Symbol: Tg(Adipoq-cre)1Evdr
• Name: transgene insertion 1, Evan Rosen
• MGI ID: MGI:4836360
• Synonyms: A-Cre, Adipoq-Cre, Adn-Cre, Tg(Adipoq-CreERT2)^tm1Jgg
• Transgene: Tg(Adipoq-cre)1Evdr Location: unknown Genetic Position: Chr9, Syntenic
• Alliance: Tg(Adipoq-cre)1Evdr page

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Adipoq-cre)1Evdr involves 1 genes/genome features (Tbx18) View all
• Tg(Adipoq-cre)1Evdr expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Adipoq (MGI:106675)

• Mutation details: To generate the Adipoq-Cre BAC transgenic mice, the 245 kb RP23-90G21 bacterial artificial chromosome (BAC) containing at least five mouse genes was modified by replacing the starting ATG and 222 bp of the adiponectin gene with the starting ATG and coding sequence of a Cre recombinase gene. Founder line 1 was analyzed. These mice express Cre recombinase effectively in white adipose tissue (WAT) and brown adipose tissue (BAT), but not in macrophages (including adipose-tissue resident macrophages, alveolar macrophages, or thioglycollate-stimulated peritoneal macrophages). The investigator reports highly efficient Cre recombinase activity, with no ectopic expression. The transgene integrated on chromosome 9 between exons 6 and 7 of the Tbx18 locus, an embryonic development gene also associated with myocardial function. No genomic rearrangements were detected in the region of the integration site, although the insertion leads to reduced Tbx18 expression in hemizygous epididymal white adipose tissue. While the targeted locus amplification mapping predicted transgene-transgene fusions had occurred between different parts of the transgene, their ddPCR analysis identified the number of copies of intact Cre recombinase as one per genome. (J:168971, J:305903)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Adipoq (mouse) Summary of all recombinase alleles driven by Adipoq. Comparative matrix view of recombinase activities Alliance mouse Adipoq gene page

Disease models

Expression

In Mice Carrying this Mutation:

312115 assay results 14 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available

References

• Original: J:168971 Eguchi J, et al., Transcriptional Control of Adipose Lipid Handling by IRF4. Cell Metab. 2011 Mar 2;13(3):249-59
• All: 484 reference(s)