Wnt3^{tm2a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Wnt3^{tm2a(EUCOMM)Wtsi}
• Name: wingless-type MMTV integration site family, member 3; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4455951
• Gene: Wnt3 Location: Chr11:103664976-103708783 bp, + strand Genetic Position: Chr11, 67.5 cM
• Alliance: Wnt3^{tm2a(EUCOMM)Wtsi} page
• IMPC: Wnt3 gene page

Mutation origin

• Mutant Cell Lines: EPD0555_1_A02, EPD0555_1_A03, EPD0555_1_A05, EPD0555_1_B03, EPD0555_1_B04, EPD0555_1_B05, EPD0555_1_C03, EPD0555_1_C04, EPD0555_1_C05, EPD0555_1_D03, EPD0555_1_E01, EPD0555_1_E02, EPD0555_1_E04, EPD0555_1_F02, EPD0555_1_F03, EPD0555_1_G01, EPD0555_1_G02, EPD0555_1_G05, EPD0555_1_G06, EPD0555_1_H01, EPD0555_1_H06
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was inserted at position 103698356 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 103699636. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Wnt3 Mutation: 25 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 4 reference(s)